Highly stable and ultra-sensitive amperometric aptasensor based on pectin stabilized gold nanoparticles on graphene oxide modified GCE for the detection of aflatoxin M1

نویسندگان

چکیده

Herein, we have elaborated a new electrochemical aptasensor for the detection of ultra-trace amounts aflatoxin M1 (AFM1) present in cow milk, with regulated low levels. To achieve AFM1, simple strategy deposition specific aptamers was elaborated, based on their grafting pectin-reduced gold nanoparticles (AuNPs) electrogenerated graphene oxide sheet modified glassy carbon electrode. The elaboration composite film confirmed by cyclic voltammetry (CV), differential pulse (DPV), impedance spectroscopy (EIS), scanning electron microscopy (SEM), and UV–visible absorption measurements. aptamer/pectin-AuNPs/GO/GCE exhibited significantly enhancement determination AFM1 20 min limit 0.2 ng/L, wide useful linear range (10–1000 ng/L). An excellent stability, high sensitivity, reproducibility as well good recovery were obtained designed aptasensor. Moreover, results tested real samples. Thus, performance preparation process can provide promising potential milk samples daily use.

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ژورنال

عنوان ژورنال: Food chemistry advances

سال: 2022

ISSN: ['2772-753X']

DOI: https://doi.org/10.1016/j.focha.2022.100068